MKK7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human MKK7 Monoclonal Antibody (Catalog # MAB3579) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MKK7 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle and tonsil

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK7

Mitogen-activated protein kinase kinase 7 (MKK7 or MAP2K7), also known as Jun kinase kinase 2 (JNKK2), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser 271 and Thr 275 by several upstream MAPK kinase kinases (MAP3Ks). MKK7 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.

Long Name

Mitogen-activated Protein Kinase Kinase 7

Alternate Names

JNKK2, MAP2K7, MKE7, PRKMK7

Entrez Gene IDs

5609 (Human); 26400 (Mouse); 363855 (Rat)

Gene Symbol

MAP2K7

UniProt

O14733

Additional MKK7 Products

Product Documents for Human MKK7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MKK7 Antibody

For research use only

Citations
Reviews

Human MKK7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3579

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MKK7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MKK7 Antibody

MKK7 in Human Tonsil.