Detects human ILT3/CD85k in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB24251F) or (B) Mouse IgG_2AFluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 2 reviews rated 4 using FAB24251F in the following applications:

Flow Cytometry (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRB4/CD85k/ILT3

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4

Alternate Names

CD85k, HM18, ILT3, LIR5

Entrez Gene IDs

11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)

Gene Symbol

LILRB4

UniProt

ABM86208

Additional LILRB4/CD85k/ILT3 Products

Product Documents for Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB24251F

Key Product Details

Species Reactivity

Validated:

Cited:

Applications