Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (FAB4869P) and either (A) Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Detection of IL-3R alpha/CD123 in THP-1 cells by Flow Cytometry

THP-1 cells were stained with Mouse Anti-Human IL-3R alpha/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-3R alpha/CD123 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes; THP-1 human acute monocytic leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

References

Moore, M.A.S. et al. (1991) Blood 72:944.
Warren, D.J. et al. (1988) J. Immunol. 140:94.
Plant M. et al. (1989) Nature 339:150.
Budel, L.M. et al. (1990) Blood 75:1439.
Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Gene Symbol

IL3RA

UniProt

P26951

Additional IL-3R alpha Products

Product Documents for Human IL-3R alpha/CD123 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-3R alpha/CD123 APC-conjugated Antibody

For research use only

Citations
Reviews

Human IL-3R alpha/CD123 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB301A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-3R alpha/CD123 APC-conjugated Antibody

Detection of IL‑3 R alpha/CD123 in Human Blood Lymphocytes by Flow Cytometry.

Detection of IL-3R alpha/CD123 in THP-1 cells by Flow Cytometry

Applications for Human IL-3R alpha/CD123 APC-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: IL-3R alpha

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional IL-3R alpha Products

Product Documents for Human IL-3R alpha/CD123 APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human IL-3R alpha/CD123 APC-conjugated Antibody