Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 676415

View all IRS2 Antibodies »

Product Specifications

Specificity

Detects human IRS2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human IRS1 or recombinant mouse IRS3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human IRS2 Alexa Fluor™ Plus 488-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: IRS2

IRS2 (insulin receptor substrate 2) is a 180-190 kDa substrate intermediate that lies between various cytokine receptors and downstream signaling molecules.  It is the primary IRS protein found in hematopoietic cells.  Depending upon the receptor, IRS2 undergoes phosphorylation, either via Tyk2 following type I IFN binding, or via JAK1 and 3 following IL-4, -7 and -15 binding.  This event allows for its subsequent association with PI-3 kinase.  Human IRS2 is 1338 amino acids (aa) in length with a predicted MW of 138 kDa.  It contains one PH (pleckstrin homology) domain (aa 16-144), followed by a PTB (phosphotyrosine-binding)domain (aa 194-298) and seven YxxM (Tyr/x/x/Met) motifs (aa 540-1075).  IRS2 is phosphorylated on multiple Ser and Tyr residues.  There are two potential splice variants. One shows a 16 aa substitution for the C-terminal Glu, while another shows an 11 aa substitution for aa 1314-1338.  Over aa 1035-1136, human IRS2 shares 86% aa identity with mouse IRS2. 

Long Name

Insulin Receptor Substrate 2

Alternate Names

insulin receptor substrate 2, IRS-2

Entrez Gene IDs

8660 (Human); 384783 (Mouse); 29376 (Rat)

Gene Symbol

IRS2

UniProt

Q9Y4H2 (Human)

Additional IRS2 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IRS2 Alexa Fluor™ Plus 488-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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