Human Integrin beta6 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4155
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta6
Gly22-Asn707
Accession # P18564
Gly22-Asn707
Accession # P18564
Specificity
Detects human Integrin beta6 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse Integrin beta6 is observed, approximately 10% cross-reactivity with recombinant human (rh) Integrin beta3 is observed, and less than 5% cross-reactivity with rhIntegrin beta5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Integrin beta6 Biotinylated Antibody
Detection of Integrin beta6 in HT‑29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin beta6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4155, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Integrin beta6 in HT‑29 Human Cell Line.
Integrin beta6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin beta6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin beta6 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: HT-29 human colon adenocarcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Integrin beta6
Sample: Recombinant Human Integrin beta6
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 6
Integrin beta6 (ITG beta6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alphaV and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta6 ECD, respectively.
Alternate Names
ITGB6
Gene Symbol
ITGB6
UniProt
Additional Integrin beta 6 Products
Product Documents for Human Integrin beta6 Biotinylated Antibody
Product Specific Notices for Human Integrin beta6 Biotinylated Antibody
For research use only
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