Human Integrin beta6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4155
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta6
Gly22-Asn707
Accession # P18564
Gly22-Asn707
Accession # P18564
Specificity
Detects human Integrin beta6 in the direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Integrin beta6 is observed and less than 5% cross-reactivity with recombinant human (rh) Integrin beta3 and rhIntegrin beta5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Integrin beta6 Antibody
Detection of Integrin beta6 in HT-29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin beta6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155, filled histogram) or isotype control antibody (5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Integrin beta6 in HT‑29 Human Cell Line.
Integrin beta6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin beta6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Integrin beta6 in HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells .
Integrin beta6 was detected in immersion fixed HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells using Sheep Anti-Human Integrin beta6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin beta6 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HT-29 human colon adenocarcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells
Sample: Immersion fixed HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human Integrin beta6
Sample: Recombinant Human Integrin beta6
Reviewed Applications
Read 1 review rated 5 using AF4155 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 6
Integrin beta6 (ITG beta6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alphaV and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta6 ECD, respectively.
Alternate Names
ITGB6
Gene Symbol
ITGB6
UniProt
Additional Integrin beta 6 Products
Product Documents for Human Integrin beta6 Antibody
Product Specific Notices for Human Integrin beta6 Antibody
For research use only
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