NorthernLights Streptavidin NL557

Detection of Rat IgG Primary Antibody by Immunocytochemistry

Detection of human Vimentin by incubating cells with 8 ug/mL of Rat anti-Human Vimentin Biotinylated monoclonal antibody (BAM2105) at room temperature for 1 hour followed by incubation with 1:5000 dilution of NorthernLights™ Streptavidin NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).