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Human IL‑28 R alpha/IFN-lambda R1 PE‑conjugated Antibody
Catalog # FAB5260P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 601106
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Product Summary
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha/IFN-lambda R1
Arg21-Ala228
Accession # Q8IU57
Arg21-Ala228
Accession # Q8IU57
Specificity
Detects human IL-28 R alpha/IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
Detection of IL‑28 R alpha/IFN-lambda R1 in Daudi Human Cell Line by Flow Cytometry.
Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of IL‑28 R alpha/IFN-lambda R1 in PBMCs by Flow Cytometry.
Peripheral blood mononuclear cells (PBMCs) either untreated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.Applications
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Daudi Human Burkitt's Lymphoma Cell Line and Peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin
Sample: Daudi Human Burkitt's Lymphoma Cell Line and Peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IL-28 R alpha/IFN-lambda R1
Product Specific Notices
For research use only
Citations for Human IL‑28 R alpha/IFN-lambda R1 PE‑conjugated Antibody (3)
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Citations are publications that use Bio-Techne products. Selected citations for Human IL‑28 R alpha/IFN-lambda R1 PE‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryVM Stone et al. (2021-01-05), Inhibition of Type III Interferon Expression in Intestinal Epithelial Cells-A Strategy Used by Coxsackie B Virus to Evade the Host's Innate Immune Response at the Primary Site of Infection? Microorganisms, 2021-09(1).
PMID: 33466313 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryL Wisgrill et al. (2019-06-18), Diminished secretion and function of IL-29 is associated with impaired IFN-? response of neonatal plasmacytoid dendritic cells J. Leukoc. Biol., 2019-00(0).
PMID: 31211458 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryY Yu et al. (2017-05-11), Hepatitis B Virus e Antigen Activates the Suppressor of Cytokine Signaling 2 to Repress Interferon Action Sci Rep, 2017-07(1):1729.
PMID: 28496097
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