Human IL-2 Antibody Best Seller

Name: Human IL-2 Antibody
Brand: R&D Systems
SKU: MAB202
Rating: 5 (1 reviews)

Catalog # MAB202 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (17)

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 5334

Product Summary for Human IL-2 Antibody

Immunogen

E. coli-derived recombinant human IL‑2
Ala21-Thr153
Accession # NP_000577

Specificity

Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IL-2 Antibody

Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 Antibody.

Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202). The ND₅₀ is typically 0.015-0.03 µg/mL.

IL‑2 in Human PBMCs.

IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human IL-2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells treated with calcium ionomycin and PMA

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells treated with PMA and Ca²⁺ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND₅₀) is typically 0.015-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB202 in the following applications:

Western Blot (1 Review)

Published Applications

Read 17 publications using MAB202 in the following applications:

Bioassay (2 Publications)
ELISA Detection (1 Publication)
Flow Cytometry (4 Publications)
ICC (1 Publication)
Neutralization (11 Publications)

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin 2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 3558 (Human); 16183 (Mouse); 396868 (Porcine); 100302458 (Rabbit); 116562 (Rat)

Gene Symbol

IL2

UniProt

NP_000577

Product Documents for Human IL-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-2 Antibody

For research use only

Citations
Reviews

Citations for Human IL-2 Antibody (17)

Showing 1 - 10 of 17 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-2 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

M Ukita et al. (2022-06-22), CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer JCI Insight, 2022-00(0).
PMID: 35552285
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Y Rodríguez- et al. (2021-12-18), Immunogenicity profile in African green monkeys of a vaccine candidate based on a mutated form of human Interleukin-15 BMC immunology, 2021-122(1):79.
PMID: 34922462
Species: Human,Mouse
Sample Types: Recombinant Protein,Serum
Applications: ELISA Detection

D Sahin et al. (2020-12-22), An IL-2-grafted antibody immunotherapy with potent efficacy against metastatic cancer Nature Communications, 2020-111(1):6440.
PMID: 33353953
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

A Güler et al. (2019-11-14), Suppressor of cytokine signaling 3 is crucial for interleukin-7 receptor re-expression after T-cell activation and interleukin-7 dependent proliferation Eur. J. Immunol., 2019-10(0).
PMID: 31621896
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

JH Kim et al. (2018-11-08), Effects of pepsin and pepstatin on reflux tonsil hypertrophy in vitro PLoS ONE, 2018-113(11):e0207090.
PMID: 30408092
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Carmenate et al. (2018-04-04), Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells J. Immunol., 2018-00(0).
PMID: 29618524
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Revu et al. (2018-03-06), IL-23 and IL-1? Drive Human Th17 Cell Differentiation and Metabolic Reprogramming in Absence of CD28 Costimulation Cell Rep, 2018-022(10):2642-2653.
PMID: 29514093
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,Neutralization

(2016-09-02), Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016-06(0):32320.
PMID: 27586092
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-07-04), Activin A programs the differentiation of human TFH cells Nat Immunol, 2016-017(8):976-84.
PMID: 27376469
Species: Human
Sample Types: Protein
Applications: Neutralization

Nata T et al. (2015-07-16), Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015-0290(37):22338-51.
PMID: 26183780
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Kim et al. (2014-05-06), Interleukin-2 alters distribution of CD144 (VE-cadherin) in endothelial cells. J Transl Med, 2014-012(0):113.
PMID: 24885155
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2012-05-10), ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood, 2012-0119(26):6268-77.
PMID: 22577174
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Hutnick NA et al. (2010-02-23), Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive. Vaccine, 2010-028(8):1932-41.
PMID: 20188249
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Withers DR et al. (2007-02-13), T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue. Blood, 2007-0109(11):4856-64.
PMID: 17299094
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

Karenko L et al. (2005-09-15), Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue. Cancer Res., 2005-065(18):8101-10.
PMID: 16166283
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Sen Y et al. (2004-06-01), Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-0112(2):274-89.
PMID: 15147571
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Herrero C et al. (2003-11-15), Reprogramming of IL-10 activity and signaling by IFN-gamma. J. Immunol., 2003-1171(10):5034-41.
PMID: 14607900

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