Human IL-17E/IL-25 Antibody

Name: Human IL-17E/IL-25 Antibody
Brand: R&D Systems
SKU: AF1258
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1258

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-17E (R&D Systems, Catalog # 1258-IL) Tyr33-Gly177 Accession # Q9H293
Applications:	Block/Neutralize, ELISA, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL-17E in direct ELISAs and Western blots. In direct ELISAs, less than 60% cross-reactivity with recombinant mouse IL‑17E is observed.

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Applications for Human IL-17E/IL-25 Antibody

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL-17E/IL-25 (Catalog # 1258-IL)

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑17E/IL‑25 Monoclonal Antibody (Catalog # MAB1258).

This product is intended for assay development on various assay platforms requiring antibody pairs.

项目	Value
Neutralization	Measured by its ability to neutralize IL‑17E/IL‑25-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.04-0.24 ug/mL in the presence of 5 ng/ml Recombinant Human IL‑17E/IL‑25.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-17E/IL-25 Antibody

	Neutralization Cell CXCL1/GRO Alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Human IL‑17E/IL‑25 Antibody. Recombinant Human IL-17E/ IL-25 induces CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha Duoset (Catalog # DY275). Under these conditions, CXCL1/GRO alpha secretion elicited by IL-17E/IL-25 is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17E/IL-25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1258). The ND₅₀ is typically 0.04-0.24 ug/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17E cDNA encodes a 177 amino acid (aa) residues precursor protein with a putative 32 aa signal peptide (3). A second isoform of human IL-17E encoding a 161 aa precursor protein also exists (4). The two isoforms differ in their signal peptide sequences. Mature human IL-17E shares 76% aa sequence identity with mature mouse IL-17E. Human IL-17E also shares from 25% to 36% aa sequence identity with the other human IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord and trachea (3). IL-17E binds and activates IL‑17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

Item	Value
References	Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155. Lee, J. et al. (2001) J. Biol. Chem. 276:1660. Hurst, S.D. et al. (2002) J. Immunol. 169:443. Pan, G. et al. (2001) J. Immunol. 167:6569.
Long Name	Interleukin 17E
Entrez Gene IDs	64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names	IL-17E, IL-25, IL17E, IL25, Interleukin-17E, interleukin 25, interleukin-25

Citations for Human IL-17E/IL-25 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-17E/IL-25 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Yuan Zhang et al. (2018-01-01), IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual ??T cells in early pregnancy. Experimental and Therapeutic Medicine, 0(0):1792-0981.
PMID: 29434775

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Human IL-17E/IL-25 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 06/09/2020

We used this antibody in an in-house ELISA along with mAb (1258) and protein (1258-IL-025) to quantify IL-17E in human serum and plasma. This combination detected IL-17E in our samples efficiently.

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Human IL-17E/IL-25 Antibody

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Applications for Human IL-17E/IL-25 Antibody

Scientific Data Examples for Human IL-17E/IL-25 Antibody

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-17E/IL-25

Citations for Human IL-17E/IL-25 Antibody (1)

Customer Reviews for Human IL-17E/IL-25 Antibody (1)

Customer Images

Human IL-17E/IL-25 Antibody

Product Documents for Human IL-17E/IL-25 Antibody

Product Datasheets

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