Human IFN-gamma Antibody Best Seller
Catalog # MAB285 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human IFN-gamma Antibody
Immunogen
Gln24-Gln166
Accession # AAP20098.1
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human IFN-gamma Antibody
IFN-gamma in Human PBMCs.
IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with either (A) Mouse Anti-Human IFN- gamma Monoclonal Antibody (Catalog # MAB285) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-gamma Antibody.
Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN-gamma Monoclonal Antibody (Catalog # MAB285). The ND50 is typically 0.02-0.06 µg/mL.Applications for Human IFN-gamma Antibody
Immunocytochemistry
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin
Intracellular Staining by Flow Cytometry
Sample: Human PBMCs treated with PMA, Ionomycin, and Monensin fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Neutralization
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma
Product Documents for Human IFN-gamma Antibody
Product Specific Notices for Human IFN-gamma Antibody
For research use only
Citations for Human IFN-gamma Antibody (25)
Citations are publications that use Bio-Techne products. Selected citations for Human IFN-gamma Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: NeutralizationR Laufer Bri et al. (2023-03-17), Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata Elife, 2023-012(0).
PMID: 36930216 -
Species: Human
Sample Types: Whole Cells
Applications: StimulationAKO Rode et al. (2022-08-19), Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor Cells, 2022-011(16).
PMID: 36010663 -
Species: Human
Sample Types: Plasma
Applications: ELISA DetectionN Smith et al. (2021-09-01), Distinct systemic and mucosal immune responses during acute SARS-CoV-2 infection Nature Immunology, 2021-00(0).
PMID: 34471264 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationF Ma et al. (2020-06-19), Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway Theranostics, 2020-010(17):7730-7746.
PMID: 32685016 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationK Schilbach et al. (2020-01-04), In the Absence of a TCR Signal IL-2/IL-12/18-Stimulated gammadelta T Cells Demonstrate Potent Anti-Tumoral Function Through Direct Killing and Senescence Induction in Cancer Cells Cancers (Basel), 2020-012(1).
PMID: 31947966 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationPA Szabo et al. (2019-10-17), Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease Nat Commun, 2019-110(1):4706.
PMID: 31624246 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayF Zhang et al. (2018-01-29), DDIT4 and Associated lncDDIT4 Modulate Th17 Differentiation through the DDIT4/TSC/mTOR Pathway J. Immunol., 2018-00(0).
PMID: 29378913 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationY Liu et al. (2017-07-31), PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2 Proc. Natl. Acad. Sci. U.S.A., 2017-00(0).
PMID: 28760986 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationMS Longhi et al. (2017-05-04), Bilirubin suppresses Th17 immunity in colitis by upregulating CD39 JCI Insight, 2017-02(9).
PMID: 28469075 -
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr(2016-08-01), Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion J Immunol, 2016-00(0).
PMID: 27481843 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization(2016-06-29), Modulation of Stat-1 in Human Macrophages Infected with Different Species of Intracellular Pathogenic Bacteria J Immunol Res, 2016-02016(0):5086928.
PMID: 27437406 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryAS Arterbery et al. (2016-04-18), Production of Proinflammatory Cytokines by Monocytes in Liver-Transplanted Recipients with De Novo Autoimmune Hepatitis Is Enhanced and Induces TH1-like Regulatory T Cells J. Immunol., 2016-0196(10):4040-51.
PMID: 27183637 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationRanzani et al. (2015-01-26), The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Nat Immunol, 2015-016(3):318-25.
PMID: 25621826 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationKongsbak M et al. (2014-05-02), Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells. PLoS ONE, 2014-09(5):e96695.
PMID: 24792400 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationChang HC et al. (2010-05-02), The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation. Nat. Immunol., 2010-011(6):527-34.
PMID: 20431622 -
Species: Human
Sample Types: Whole Cells
Applications: Differentiation,DifferentiationChang HC et al. (2009-04-09), Impaired development of human Th1 cells in patients with deficient expression of STAT4. Blood, 2009-0113(23):5887-90.
PMID: 19359411 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationSeshasayee D et al. (2007-12-01), In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation. J. Clin. Invest., 2007-1117(12):3868-3878.
PMID: 18060034 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationPontikoglou C et al. (2006-10-01), Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia. Exp. Hematol., 2006-134(10):1312-22.
PMID: 16982324 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationEbert EC et al. (2006-08-17), Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006-0241(1):7-13.
PMID: 16914126 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCMethe H et al. (2005-07-01), Peripheral expansion of circulating T-helper 1 cells predicts coronary endothelial dysfunction after cardiac transplantation. J. Heart Lung Transplant., 2005-024(7):833-40.
PMID: 15982610 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationMiranda-Carus ME et al. (2004-07-15), IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. J. Immunol., 2004-0173(2):1463-76.
PMID: 15240743 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationHerrero C et al. (2003-11-15), Reprogramming of IL-10 activity and signaling by IFN-gamma. J. Immunol., 2003-1171(10):5034-41.
PMID: 14607900 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationDurali D et al. (2003-07-31), In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment. Blood, 2003-0102(12):4084-9.
PMID: 12893768 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2002-06-01), Cutting edge: immune cells as sources and targets of the IL-10 family members? J. Immunol., 2002-0168(11):5397-402.
PMID: 12023331 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationOng PY et al. (2002-01-01), Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis. J. Immunol., 2002-0168(1):505-10.
PMID: 11751999
There are no citations that match your criteria.
Customer Reviews for Human IFN-gamma Antibody (4)
Customer Images
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Peripheral blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 06/17/2022
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Application: Block/NeutralizeSample Tested: Cell Culture MediaSpecies: HumanVerified Customer | Posted 04/28/2021
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 03/11/2019
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/09/2019
There are no reviews that match your criteria.
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