HLA-DRA Antibody was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 5ug/mL at 32° Celsius for2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of HLA-DRA in Human Appendix via seqIF™ staining on COMET™

HLA-DRA Antibody was detected in immersion fixed paraffin-embedded sections of human Appendix using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 5ug/mL at 32° Celsius for2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of HLA-DRA in Human Hodgkin Lymphoma via seqIF™ staining on COMET™

HLA-DRA Antibody was detected in immersion fixed paraffin-embedded sections of human Hodgkin Lymphoma using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 5ug/mL at 32° Celsius for2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Human HLA-DR by Western Blot.

Western Blot shows lysates of HDLM-2 human Hodgkin’s lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human HLA-DR Monoclonal Antibody (Catalog # MAB11555) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HLA-DR at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of HLA-DR in Human Tonsil.

HLA-DR was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA-DR Monoclonal Antibody (Catalog # MAB11555) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human HLA-DR by Simple Western^TM.

Simple Western shows lysates of HDLM-2 human Hodgkin’s lymphoma cell line, loaded at 1 mg/ml. A specific band was detected for HLA-DR at approximately 42 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HLA-DR Monoclonal Antibody (Catalog # MAB11555). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Applications for Human HLA-DR Antibody

Application

Recommended Usage

COMET

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Multiplex Immunofluorescence

1 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil tissue

Simple Western

20 µg/mL
Sample: HDLM-2 human Hodgkin's lymphoma cell line

Western Blot

2 µg/mL
Sample: HDLM-2 human Hodgkin's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HLA-DR

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4⁺ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Long Name

Major Histocompatibility Complex Class II DR

Alternate Names

HLA-DRA, HLADR, MHC Class II DR

Entrez Gene IDs

3122 (Human)

Gene Symbol

HLA-DRA

UniProt

P01903

Additional HLA-DR Products

Product Documents for Human HLA-DR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human HLA-DR Antibody

For research use only

Citations
Reviews

Human HLA-DR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11555

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all HLA-DR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human HLA-DR Antibody

Detection of HLA-DRA in Human Tonsil via seqIF™ staining on COMET™