Human FKBP52 Antibody

Detection of Human FKBP52 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human FKBP52 Monoclonal Antibody (Catalog # MAB4095) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, 5 ng recombinant human FKBP38, FKBP51, and FKBP52 were included. A specific band for FKBP52 was detected at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of FKBP52 by Western Blot

Experimental design and confirmation of GFP and FKBP52 overexpression in the hippocampus.a Timeline of bilateral hippocampal AAV injections and experiments performed in WT and rTg4510 mice. b Representative images of AAV9-GFP and AAV9-FKBP52 hippocampal sections after 3 months of viral expression. c Representative Western blots and quantification of GFP and FKBP52 overexpression in these animals. Wild-type mice [n = 16], rTg4510 mice [n = 16]. Protein levels were normalized to beta-Actin and analyzed by unpaired t test, where statistical significance is represented by **p < 0.01 and ***p < 0.001. Scale bar represents 200 µm; inset scale represents 20 µm. AAV9 adeno-associated virus serotype 9, GFP green fluorescent protein, WT wild-type, HPC hippocampus. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33941782), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FKBP52 by Immunohistochemistry

Overexpression of FKBP52 promotes neurotoxicity in aged wild-type mice. (a) Representative images from hippocampal neurons stained with NeuN (brown) and cresyl violet (purple). These hippocampal slices correspond to AAV9-mCherry, AAV9-Aha1, and AAV9-FKBP52 injected wild-type mice. Quantification of CA1 hippocampal (b) neuronal density and (c) volume using unbiased stereology from these animals. N = 6/AAV. Results represent the standard error of the mean (± SEM). Data was analyzed by a one-way ANOVA followed by Tukey post-hoc test. *p < 0.05 and **p < 0.01 is considered statistical difference in neuronal loss. Scale bars = 100 µm and inset scale represents 10 µm. CV, cresyl violet; CA1, Cornu ammonis subfield 1; HPC, hippocampus Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33832539), licensed under a CC-BY license. Not internally tested by R&D Systems.