HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FATP2 Mono-clonal Anti-body (Catalog # MAB4659, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Anti-body (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human FATP2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FATP2

Fatty Acid Transport Protein 2 (FATP2), also known as SLC27A2 (solute carrier family 27, member 2), is a multipass transmembrane protein that participates in the transport and metabolism of long chain fatty acids. It has acyl-CoA synthase activity and contains an AMP-binding motif. FATP2 localizes to the endoplasmic reticulum and is predominantly expressed in the liver and kidney. Human FATP2 shares 82% aa sequence identity with mouse and rat FATP2.

Long Name

Fatty Acid Transport Protein 2

Alternate Names

ACSVL1, FACVL1, SLC27A2, THCA-CoA ligase, VLACS, VLCS

Entrez Gene IDs

11001 (Human); 26458 (Mouse); 65192 (Rat)

Gene Symbol

SLC27A2

UniProt

O14975

Additional FATP2 Products

Product Documents for Human FATP2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FATP2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FATP2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4659

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FATP2 Antibody

Detection of FATP2 in HepG2 Human Cell Line by Flow Cytometry.