Human CXCR6 PE-conjugated Antibody

Detection of CXCR6 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 150 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days were stained with Mouse Anti-Human CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB699P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (IC0041P). View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR6 in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human CXCR6 and eGFP (A) vs irrelevant and eGFP (B) were stained with Mouse Anti-Human CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB699P) at 37°C. View our protocol for Staining Membrane-associated Proteins.

Detection of Human CXCR6 by Flow Cytometry

CXCR6 and CXCL16 expression in LuCa cell lines(A) mRNA levels by semiquantitative RT-PCR. The copies of CXCR6 and CXCL16 transcripts are expressed relative to copies of 18S rRNA. Values are mean ± SEM from 3 independent experiments. *p ≤ 0.05, ***p ≤ 0.001 as compared to SCC (NCI-H520). (B) Flow cytometry analysis of CXCR6 and trans-membrane CXCL16 in (i) - SCC (NCI-H520) cells and (ii) - AC (NCI-H2126) cells. Grey dots represent isotype controls for PE and APC antibody and black dots represent CXCR6-PE and CXCL16-APC in SCC (NCI-H520) and AC (NCI-H2126). CXCR6 and CXCL16 both are expressed on ~82.9% (Q2) SCC cells; 5.41% (Q1) express only CXCR6 and only CXCL16 is expressed by 9.08% (Q3) of SCC cells. In AC cells, 61.7% of the population express both receptor and ligand (Q2) whereas, 11.1 (Q1) and 16.2% (Q3) cells express only CXCR6 and CXCL16, respectively. c) Levels of soluble CXCL16 in LuCa supernatant. Values are mean ± SEM from 3 independent experiments. ***p ≤ 0.0001 compared to NCI-H520. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.3194), licensed under a CC-BY license. Not internally tested by R&D Systems.