HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP (A) vs irrelevant transfectant (B) were stained with Mouse Anti-Human Complement Component C3aR Monoclonal Antibody (Catalog # MAB5995) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of Complement Component C3a R in Human Monocytes by Flow Cytometry.

Human blood-derived monocytes were stained with Mouse Anti-Human Complement Component C3a R Monoclonal Antibody (Catalog # MAB5995, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Applications for Human Complement Component C3aR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embyonic kindey cell line transfected with human C3aR and eGFP or irrelevant transfectant and Human blood‑derived monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C3aR

Complement Component 3a receptor (C3a R) is an ~90 kDa G-protein coupled 7-transmembrane sulfated glycoprotein expressed on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells. Engagement of C3a R by C3a activates mast cells and neutrophils, triggering degranulation in airways during asthmatic allergen challenges. It assists in Th2-type inflammatory reactions and stimulates leukocyte chemotaxis. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

Long Name

Complement Component C3a Receptor

Alternate Names

AZ3B, C3AR, C3AR1, C3R1, Complement Component C3a R

Entrez Gene IDs

719 (Human); 12267 (Mouse)

Gene Symbol

C3AR1

UniProt

Q16581

Additional Complement Component C3aR Products

Product Documents for Human Complement Component C3aR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Complement Component C3aR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry