Human CELSR3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7278

R&D Systems, part of Bio-Techne
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MAB7278
MAB7278-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 763103

View all CELSR3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CELSR3
Val531-Ser711
Accession # Q9NYQ7

Specificity

Detects human CELSR3 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CELSR3 Antibody

Detection of Human CELSR3 antibody by Western Blot.

Detection of Human CELSR3 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR3. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human CELSR3 Monoclonal Antibody (Catalog # MAB7278) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for CELSR3 at approximately 260 and 400 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human CELSR3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human CELSR3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CELSR3

CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 98% aa sequence identity with mouse CELSR3.

Long Name

Cadherin, EGF LAG Seven-pass G-type Receptor 3

Alternate Names

CDHF11, EGFL1, HFMI1, MEGF2, RESDA1

Entrez Gene IDs

1951 (Human); 107934 (Mouse); 83466 (Rat)

Gene Symbol

CELSR3

UniProt

Q9NYQ7 (Human)

Additional CELSR3 Products

Product Documents for Human CELSR3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CELSR3 Antibody

For research use only

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