Human CD94 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1058
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready
Cited:
Neutralization, Flow Cytometry, Functional Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 131412
Product Specifications
Immunogen
BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A
Specificity
Recognizes human CD94 both in its homodimeric form and as a heterodimer with either NKG2A or NKG2C.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human CD94 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
HLA-E tetramers at 3 μg/mL were incubated with BaF/3 cells transfected with hNKG2C/CD94, and binding was monitored by flow cytometry. A 15 minute pre-incubation of the transfected cells with this antibody at a concentration of 17 μg/mL inhibited 95% of the HLA-E tetramer binding.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood CD56+ natural killer cells
Sample: Human whole blood CD56+ natural killer cells
Reviewed Applications
Read 2 reviews rated 2 using MAB1058 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD94
The type-II transmembrane glycoprotein CD94 covalently associates with C-type lectins of the NKG2 family to yield heterodimers important in NK cell recognition of class I MHC molecules. CD94/NKG2A complexes are also found on a subset of CD8+ T cells. Expression of CD94/NKG2 heterodimers may regulate cell survival and effector functions. There are two alternatively spliced variants of CD94 that differ by 31 amino acids encoded by exon 2.
Alternate Names
CD94, KLRD1
Gene Symbol
KLRD1
Additional CD94 Products
Product Documents for Human CD94 Antibody
Product Specific Notices for Human CD94 Antibody
For research use only
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