Human CD63 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC5048P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 460305
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human CD63
Met1-Met238
Accession # P08962
Met1-Met238
Accession # P08962
Specificity
Stains human CD63 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD63 PE-conjugated Antibody
Detection of CD63 in Human PBMC monocytes by Flow Cytometry.
PBMC monocytes were stained with Mouse Anti-Human CD63 PE-conjugated Monoclonal Antibody (Catalog # IC5048P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD63 in NS0 Mouse Cell Line Transfected with Human CD63 and eGFP by Flow Cytometry.
NS0 mouse myeloma cell line transfected with Human CD63 and eGFP was stained with Mouse Anti-Human CD63 PE-conjugated Monoclonal Antibody (Catalog # IC5048P). Quadrant markers were set based on control antibody staining (MAB002). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.Applications for Human CD63 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: PBMC monocytes
Sample: PBMC monocytes
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: NS0 mouse myeloma cell line transfected with Human CD63 and eGFP were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: NS0 mouse myeloma cell line transfected with Human CD63 and eGFP were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD63
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.
Alternate Names
CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30
Gene Symbol
CD63
UniProt
Additional CD63 Products
Product Documents for Human CD63 PE-conjugated Antibody
Product Specific Notices for Human CD63 PE-conjugated Antibody
For research use only
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