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Human CD4 Antibody

Catalog # AF-379-NA | R&D Systems, Inc. a Bio-Techne Brand
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AF-379-NA
AF-379-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human CD4 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Lys26-Trp390
Accession # P01730

Specificity

Detects human CD4 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD4 Antibody

CD4 antibody in Human T Cells by Immunocytochemistry (ICC).

CD4 in Human T Cells.

CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
CD4 antibody in Human PBMCs by Immunocytochemistry (ICC).

CD4 in Human PBMCs.

CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD4 antibody in Human Tonsil Tissue by Immunohistochemistry (IHC-P).

CD4 in Human Tonsil Tissue.

CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.

Applications for Human CD4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human T cells and human peripheral blood mononuclear cells (PBMCs)

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil tissue

Simple Western

10 µg/mL
Sample: Human PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cell line

Western Blot

1 µg/mL
Sample: PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.7 using AF-379-NA in the following applications:

Published Applications

Read 14 publications using AF-379-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References

  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

Product Documents for Human CD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD4 Antibody

For research use only

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FAQs for Human CD4 Antibody

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  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A: Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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