| Specificity: |
Detects recombinant human CD31/PECAM-1 protein in Direct ELISA.
Applications for Human CD31/PECAM-1 Antibody
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Recommended Concentration
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Sample |
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Western Blot |
2 µg/mL |
HUVEC human umbilical vein endothelial cells |
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Simple Western |
5 µg/mL |
HUVEC human umbilical vein endothelial cells |
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Immunohistochemistry |
0.05-10 µg/mL |
Immersion fixed paraffin-embedded sections of human liver tumor and normal human liver |
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Multiplex Immunofluorescence |
0.1 µg/mL |
Immersion fixed paraffin-embedded sections of human Lymph Node |
Scientific Data Examples for Human CD31/PECAM-1 Antibody
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Multiplex Immunofluorescence
Detection of CD31 in Human Lymph Node via seqIF™ staining on COMET™
CD31 was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Mouse Anti-Human CD31, Monoclonal Antibody (Catalog #MAB11666) at 0.1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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Western Blot
Detection of Human CD31/PECAM‑1 by Western Blot.
Western Blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Immunohistochemistry
Detection of CD31/PECAM‑1 in Human Liver Tumor.
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver tumor using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Immunohistochemistry
Detection of CD31/PECAM‑1 in Human Liver.
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Simple Western
Detection of Human CD31/PECAM‑1 by Simple WesternTM.
Simple Western shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.5 mg/ml. A specific band was detected for CD31/PECAM‑1 at approximately 168 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from hybridoma culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human CD31/PECAM-1 Antibody
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