Human CD20 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4225P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 396444
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human MS4A1/CD20
Met1-Pro297
Accession # P11836
Met1-Pro297
Accession # P11836
Specificity
Detects human MS4A1/CD20. Stains human MS4A1/CD20 transfectants but not irrelevant transfectants in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD20 PE-conjugated Antibody
Detection of MS4A1/CD20 in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP was stained with and either (A) Mouse Anti-Human MS4A1/CD20 PE-conjugated Monoclonal Antibody (Catalog # FAB4225P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Detection of MS4A1/CD20 in Human Peripheral Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human MS4A1/CD20 PE-conjugated Monoclonal Antibody (Catalog # FAB4225P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD20 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes and HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP
Sample: Human peripheral blood lymphocytes and HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD20
CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanning protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa identity between human and mouse CD20.
Long Name
Cluster of Differentiation 20
Alternate Names
B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7
Entrez Gene IDs
Gene Symbol
MS4A1
UniProt
Additional CD20 Products
Product Documents for Human CD20 PE-conjugated Antibody
Product Specific Notices for Human CD20 PE-conjugated Antibody
For research use only
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