Human AMPK beta1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11642
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1092531
Product Specifications
Immunogen
E. coli-derived recombinant human AMPKbeta1
Met1-Ile270
Accession # Q9Y478
Met1-Ile270
Accession # Q9Y478
Specificity
Detects recombinant human AMPK beta1 protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human AMPK beta1 Antibody
Detection of Human AMPK beta1 by Western Blot.
Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human AMPK beta1 Monoclonal Antibody (Catalog # MAB11642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK beta1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of AMPK beta1 in Human Kidney.
AMPK beta1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human AMPK beta1 Monoclonal Antibody (Catalog # MAB11642) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human AMPK beta1 by Simple WesternTM.
Simple Western shows lysates of HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for AMPK beta1 at approximately 52 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human AMPK beta1 Monoclonal Antibody (Catalog # MAB11642). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human AMPK beta1 Antibody
Application
Recommended Usage
Immunohistochemistry
0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Simple Western
10 µg/mL
Sample: HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line
Sample: HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AMPK beta 1
AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3), with all 12 combinations able to form complexes. The beta1 subunit is expressed at higher levels than the beta2 subunit in liver, while beta2 is more abundant than beta1 in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase complex as a therapeutic target in heart disease, obesity, and diabetes.
Long Name
AMP-activated Protein Kinase beta 1
Alternate Names
PRKAB1
Gene Symbol
PRKAB1
UniProt
Additional AMPK beta 1 Products
Product Documents for Human AMPK beta1 Antibody
Product Specific Notices for Human AMPK beta1 Antibody
For research use only
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