HASMC human human aortic smooth muscle cells was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of ACLP in Molt-4 Human Cells by Flow Cytometry.

Molt-4 human human cell line was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human ACLP Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HASMC human human aortic smooth muscle cells and Molt-4 human cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACLP

Aortic carboxypeptidase-like protein (ACLP), also known as adipocyte enhancer binding protein (AEBP1), is a 175 kDa molecule that contains a discoidin-like domain (aa 383-540) and a peptidase-like domain (aa 560-942). It is expressed in proliferating adipocytes and represses the transcription of FABP4. ACLP promotes macrophage cholesterol retention, the formation of foam cells, and inflammatory responses. It is also an extracellular matrix-associated protein found in fibrotic tissues including vascular smooth muscle. An alternately spliced isoform lacks aa 1-457 and has an altered sequence at its N-terminus. Over aa 345-1158, human ACLP shares 88% aa sequence identity with mouse and rat ACLP.

Long Name

Aortic Carboxypeptidase-like Protein

Alternate Names

AEBP1

Entrez Gene IDs

165 (Human)

Gene Symbol

AEBP1

UniProt

Q8IUX7

Additional ACLP Products

Product Documents for Human ACLP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ACLP Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ACLP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6425

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ACLP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ACLP Antibody

Detection of ACLP in HASMC Human Cells by Flow Cytometry.