Human PBMC were stained with (A) Rat Anti-Human ACE-2 PE-conjugated Monoclonal Antibody (Catalog # FAB9334P) or (B) Rat IgG2b Isotype Control Antibody (IC013P) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human ACE-2 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human PBMCs

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ACE-2

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through thesFAB9334Pe functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12). Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

References

Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
Yan, et al. (2020) Science 367:1444.
Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
Kuba, K. et al. (2005) Nature Med. 11:875.
Hoffman, M. et al. (2020) Cell. 181:1.
Wrapp, et al. (2020) Science 367:1260.
Imai, Y. et al. (2005) Nature 436:112.
Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
Jia, H. et al. (2016) Shock. 46:239.
Cole-Jeffrey, C.T. et al. (2015) J. Cadiovasc. Pharmacol. 66:540.
Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
Crackower, M.A. et al. (2002) Nature 417:822.

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Gene Symbol

ACE2

UniProt

Q9BYF1

Additional ACE-2 Products

Product Documents for Human ACE-2 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ACE-2 PE-conjugated Antibody

For research use only

Citations
Reviews

Human ACE-2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9334P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ACE-2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ACE-2 PE-conjugated Antibody

Detection of ACE-2 in Human PBMC by Flow Cytometry.

Applications for Human ACE-2 PE-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: ACE-2

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional ACE-2 Products

Product Documents for Human ACE-2 PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human ACE-2 PE-conjugated Antibody