Fibulin 1 Antibody (CL0337) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52918

Novus Biologicals, part of Bio-Techne
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NBP2-52918-25ul
NBP2-52918
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0337

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Fibulin 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P23142, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Fibulin 1 Antibody (CL0337) - BSA Free (NBP2-52918) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Fibulin 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Fibulin 1 Antibody (CL0337) - BSA Free

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining in human cervix, uterine and liver tissues. Corresponding FBLN1 RNA-seq data are presented for the same tissues.
Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918]

Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918]

Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-FBLN1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918]

Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918]

Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Lane 1: Marker [kDa] Lane 2: Human plasma
View 6 more images

Applications for Fibulin 1 Antibody (CL0337) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibulin 1

Fibulin 1 is a matricellular glycoprotein component of the elastic fiber core in connective tissues. It self-aggregates and additionally binds to several extracellular matrix and cellular proteins including Aggrecan, Versican, Fibronectin, ECM1, NOV/CCN3, and pro-HB-EGF. Fibulin 1c enhances ADAMTS-1 mediated cleavage of Aggrecan and Versican and can itself be cleaved by neutrophil elastase or MMP-13. It functions as a bridge between Fibrinogen and Fibrin, enabling Fibrin attachment to the platelet cell surface via Integrin alpha 2 beta 3. Fibulin 1 inhibits cardiomyocyte proliferation and Fibronectin-mediated cell adhesion and can promote bone formation.

Alternate Names

FBLN1, FIBL1

Gene Symbol

FBLN1

Additional Fibulin 1 Products

Product Documents for Fibulin 1 Antibody (CL0337) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fibulin 1 Antibody (CL0337) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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