Complement Component C3a Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # AF3677CL1
![Complement Component C3a Antibody [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/af3677cl1_goat-complement-component-c3a-pab-corafluor-1-12920251041456.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
CoraFluor 1
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant human C3a
Ser672-Arg748
Accession # P01024
Ser672-Arg748
Accession # P01024
Specificity
Detects human Complement Component C3a in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Complement Component C3a Antibody [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Complement Component C3a Antibody [CoraFluor™ 1]
Application
Recommended Usage
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Complement Component C3a
Complement Component C3a is an anaphylotoxin polypeptide that is proteolytically generated from C3. C3a formation is common to all three pathways of complement activation: classical (antibody-mediated), lectin and alternative. It binds C3a R on myeloid peripheral blood leukocytes, activated lymphocytes, endothelial and internal organ epithelial cells. It activates mast cells, neutrophils, and macrophages, enhances Th2 inflammatory reactions, promotes Th1 cell maturation, and inhibits Treg differentiation. It also stimulates leukocyte chemotaxis and smooth muscle contraction. Removal of the C-terminal arginine generates C3adesArg/ASP that binds the C5L2 receptor (GPR77) and stimulates triglyceride synthesis in adipocytes.
Alternate Names
Anaphylatoxin, C3
Gene Symbol
C3
Additional Complement Component C3a Products
Product Documents for Complement Component C3a Antibody [CoraFluor™ 1]
Product Specific Notices for Complement Component C3a Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...