BoNT-E Heavy Chain Antibody (785208) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB7135CL1
![BoNT-E Heavy Chain Antibody (785208) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab7135cl1_mouse-bont-e-heavy-chain-mab-785208-corafluor-1-221220251221293.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
C. botulinum
Applications
Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2B Clone # 785208
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant C. botulinum BoNT-E Heavy Chain
Lys845-Lys1251
Accession # P30995
Lys845-Lys1251
Accession # P30995
Reactivity Notes
C. botulinum
Specificity
Detects C. botulinum BoNT-E Heavy Chain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant Heavy Chains from BoNT-A or BoNT-G is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for BoNT-E Heavy Chain Antibody (785208) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for BoNT-E Heavy Chain Antibody (785208) [CoraFluor™ 1]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB7135. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: BoNT-E Heavy Chain
There are seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction.
E. coli-expressed recombinant BoNT light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.
Long Name
Botulinum Neurotoxin Type E Heavy Chain
Alternate Names
BoNTE Heavy Chain, Bontoxilysin-E
Additional BoNT-E Heavy Chain Products
Product Documents for BoNT-E Heavy Chain Antibody (785208) [CoraFluor™ 1]
Product Specific Notices for BoNT-E Heavy Chain Antibody (785208) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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