BMP-2 Antibody - BSA Free

Western Blot: BMP-2 AntibodyBSA Free [NBP1-19751]

BMP-2-Antibody---BSA-Free-Western-Blot-NBP1-19751-img0010.jpg

Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751]

Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - Paraffin-embedded human heart tissue.

Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751]

Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Analysis of BMP2 in mouse heart using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751]

BMP-2-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-19751-img0011.jpg

Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] -

Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 & satellite cells activity we performed both western blot & immune-gold analysis. In muscle biopsies of OA patients we found several large satellite cells syncytium (arrows) (a). High magnifications show immunolabeling for BMP-2 in perinuclear areas (squares) (40.000x) (b). BMP-2 molecules were expressed in the satellite cells syncytium cytoplasm (squares) & next to mitochondria (insert) (40.000 & 60.000x) (c). Numerous BMP-2 molecules were found in the fiber body (40.000x) (d). OP patients do not express BMP-2 in satellite cells (10.000x & 40.000x) ((e)-(f)). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] -

Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] -

Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] -

Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD macrophages & (b) splenic macrophages were stained with anti-BMP2 antibody (green) & counterstained with DAPI (blue), demonstrating that nBMP2 is expressed & localized to the nucleus in wild type macrophages, & that nuclear translocation of nBMP2 is inhibited in mutant macrophages. BMP2 labeling within the cytoplasm is present in both wild type & mutant cells as expected, because the targeted mutation allows translation of nBMP2 in the cytoplasm but inhibits nuclear translocation, & it allows normal synthesis & secretion of conventional BMP2. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30700748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] -

Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD macrophages & (b) splenic macrophages were stained with anti-BMP2 antibody (green) & counterstained with DAPI (blue), demonstrating that nBMP2 is expressed & localized to the nucleus in wild type macrophages, & that nuclear translocation of nBMP2 is inhibited in mutant macrophages. BMP2 labeling within the cytoplasm is present in both wild type & mutant cells as expected, because the targeted mutation allows translation of nBMP2 in the cytoplasm but inhibits nuclear translocation, & it allows normal synthesis & secretion of conventional BMP2. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30700748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] -

Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] -

Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, & CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field (HPF), whereas western blot for BMP-2 was evaluated by lines densitometry. (a) Notably, we found that OA muscle biopsies showed a significantly higher number of BMP-2-positive fibers (293.0 ± 35.4) as compared with muscle of OP patients (162.1 ± 33.7) (p < 0.0001). (b) Western blot lines show a higher expression of BMP-2 in OA patients compared to OP group. (c) The number of myostatin positive fibers in OP patients was significantly higher compared to OA group (p < 0.0200). (d) CD44 expression shows a significantly different rate of CD44 positive cells in OA group as compared with OP (p < 0.0020). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] -

Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP2 is a paracrine factor in WAT & regulates adipogenic differentiation in a depot-specific manner. a, b, c Western blots & summary graphs of BMP2 & alpha-tubulin expression in abdominal (A; black) & gluteal (G; white) AT (A; n = 5 women; 37–44 years, BMI 24.1–27.1 kg/m2), & BMP2 & beta-actin expression in proliferating (b) & differentiated (c) immortalised preadipocytes (n = 3). Relative protein expression data presented as mean ± SEM with individual data points overlaid (grey dots); data analysed by paired t-test. dBMP2 mRNA expression in primary abdominal (solid line) & gluteal (dashed line) preadipocytes during 3 days of proliferation (P) followed by 14 days of differentiation (d) (n = 8; 4 men, 4 women; age 32–44 years, BMI 20.5–26 kg/m2). mRNA expression normalised to PPIA. Data analysed by repeated measures ANOVA. e, f, g, h Proliferation analysis (e) in immortalised preadipocytes after 48 h culture in growth medium supplemented with BMP2 (n = 4). Triacylglycerol (TAG) accumulation (f) & PPARG2 (g) & ADIPOQ (h) mRNA expression in immortalised abdominal (black) & gluteal (white) preadipocytes after 14 day culture in BMP2-supplemented adipogenic medium (n = 3). mRNA expression normalised to 18 s. All data presented as mean fold change relative to vehicle ± SEM with individual data points overlaid (grey dots). Data analysed by ANOVA with Bonferroni post-hoc test (e, f, g, h); *p < 0.05, **p < 0.01, ***p < 0.001, compared to same depot vehicle; ‡p < 0.001, compared to same depot 5 ng/ml treatment Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31324879), licensed under a CC-BY license. Not internally tested by Novus Biologicals.