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Alkaline Phosphatase, Intestinal Antibody [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-7329

Novus Biologicals, part of Bio-Techne
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NB120-7329-0.1mg

Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Alkaline Phosphatase, Intestinal, Intestinal [Calf Intestine] (Uniprot: P19111)

Reactivity Notes

Cross reactivity against Alkaline Phosphatase, Intestinal from other tissues and species may occur but have not been specifically determined.

Localization

Attached to the membrane by a GPI-anchor.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]

Applications for Alkaline Phosphatase, Intestinal Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000-1:50000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:5000
Application Notes
This product has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Intestinal

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Long Name

Alkaline Phosphatase, Intestinal Type

Alternate Names

alkaline phosphatase, intestinal, alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme

Gene Symbol

ALPI

UniProt

Additional Alkaline Phosphatase, Intestinal Products

Product Documents for Alkaline Phosphatase, Intestinal Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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