Alkaline Phosphatase, Intestinal Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7329

Key Product Details
Species Reactivity
Bovine
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Alkaline Phosphatase, Intestinal, Intestinal [Calf Intestine] (Uniprot: P19111)
Reactivity Notes
Cross reactivity against Alkaline Phosphatase, Intestinal from other tissues and species may occur but have not been specifically determined.
Localization
Attached to the membrane by a GPI-anchor.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]
Applications for Alkaline Phosphatase, Intestinal Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase, Intestinal
Long Name
Alkaline Phosphatase, Intestinal Type
Alternate Names
alkaline phosphatase, intestinal, alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme
Gene Symbol
ALPI
UniProt
Additional Alkaline Phosphatase, Intestinal Products
Product Documents for Alkaline Phosphatase, Intestinal Antibody [HRP]
Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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