Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7328
Key Product Details
Species Reactivity
Bovine
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Alkaline Phosphatase, Intestinal, Intestinal [Calf Intestine] (Uniprot: P19111)
Reactivity Notes
Cross reactivity against Alkaline Phosphatase, Intestinal from other tissues and species may occur but have not been specifically determined.
Localization
Attached to the membrane by a GPI-anchor.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:4000-1:20000
Western Blot
1:500-1:2000
Application Notes
This product has been assayed against 1.0 ug of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase, Intestinal
Long Name
Alkaline Phosphatase, Intestinal Type
Alternate Names
alkaline phosphatase, intestinal, alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme
Entrez Gene IDs
248 (Human)
Gene Symbol
ALPI
UniProt
Additional Alkaline Phosphatase, Intestinal Products
Product Documents for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free
Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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