FlowTACS Flow Cytometry Apoptosis Detection Kit

R&D Systems, part of Bio-Techne | Catalog # 4817-60-K

Identification and quantitation of fixed apoptotic cells in suspension by Flow Cytometry.
Discontinued Product
4817-60-K has been discontinued. View all TUNEL Assays products.

Key Product Details

Features

Provides an in situ labeling technology to identify and quantitate cultured apoptotic cells by TUNEL-based and flow cytometry.

Key Benefits

  • Identification and quantitation of fixed apoptotic cells in suspension by flow cytometry.
  • Allows bi-color and tri-color analysis.
  • Designed for use with fixed cells.
  • Includes exclusive non-enzymatic Cytonin permeabilization reagent.
  • Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Species

Multi-Species

Product Summary for FlowTACS Flow Cytometry Apoptosis Detection Kit

Why Use the FlowTACS Flow Cytometry Apoptosis Detection Kit?
FlowTACS also provides flexibility in selection of fluorophores that are compatible with your research design. The kit allows multi-color labeling in conjunction with experiment-specific antibodies.

Scientific Data Images for FlowTACS Flow Cytometry Apoptosis Detection Kit

FlowTACS Flow Cytometry Apoptosis Detection Kit_4817-60-K

Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes.

Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.

Kit Contents for FlowTACS Flow Cytometry Apoptosis Detection Kit

  • Streptavidin-FITC
  • TACS-Nuclease
  • TACS-Nuclease Buffer
  • /TACS 2 TdT dNTP
  • TdT Enzyme
  • 50x Manganese Cation
  • 10X TdT Labeling Buffer
  • 10X TdT Stop Buffer
  • PI/RNase
  • Cytonin

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for FlowTACS Flow Cytometry Apoptosis Detection Kit

Product Datasheets

Certificate of Analysis

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