Application Brief 1015: ERK1/2 NanoPro Assay

Extracellular signal-regulated kinases (ERK) or classical MAP kinases are widely expressed intracellular signaling molecules involved in regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli (including growth factors, cytokines, virus infection, ligands for heterotrimeric G protein-coupled receptors, transforming agents, and carcinogens) activate the ERK pathway. We show an example of ERK phosphorylation in MCF10A cells in response to treatment with epidermal growth factor (EGF).