Advanced Western Blotting Solutions for Cell & Gene Therapy
One Platform, 6+ Assays for CQAs
The Simple Western™ Advantage
Sensitivity. Conserve your precious AAV sample with purity assays that are more sensitive than SYPRO Ruby.
Quantitation. Quantitative protein measurements with a 4-log dynamic range.
Reproducibility. Eliminate the variation of traditional Western blots with CVs consistently below 15-20%.
High Throughput. Up to 96 data points in a single overnight run, all in a fully automated fashion.
Method Transferability. Compass for Simple Western™ produces digital run files containing assay conditions and data, allowing for easy analysis, transfer, and archiving between sites or from sponsors to CROs. 21 CFR Part 11-compliant and GMP-ready.
Companies at the forefront of cell & gene therapy commercialization are adopting the multi-attribute analysis technology of Simple Western to advance quality control (QC) of AAV-mediated gene therapy products.
See how the Cell and Gene Therapy Catapult (CGTC) uses highly-specific antibodies manufactured by PROGEN with fully automated Simple Western assays for multi-attribute analysis of AAV capsids during product purification.
All-in-One Lentiviral Assays for Cell Therapy
When it comes to viral vector characterization for cell and gene therapies, Simple Western isn't just limited to AAVs. With Jess, you get multi-attribute analysis for lentivirus as well, including titer, purity, capsid content, stability, transduction analysis, and more.
Single-Cell Westerns on Milo measure protein expression in thousands of single cells per run. Their high sensitivity combined with the ability to use conventional Western blotting antibodies makes them particularly well-suited for quantifying gene therapy efficacy and tracking stem cell differentiation.
Milo Advances Gene Therapies
Milo's Single-Cell Westerns are used in clinical trials aimed at advancing gene therapies for Sickle Cell Disease and other hemoglobinopathies. Milo resolves hemoglobin subunits in single red blood cells, enabling researchers to track the efficacy of sickle cell gene therapies.
Identify and Quantify Neural Subtypes
Milo can identify and quantify neural subtypes in a heterogeneous neural sample, and monitor the differentiation of iPSCs into neurons, astrocytes, and oligodendrocytes using our research-grade or GMP differentiation reagents.
Webinars in Cell & Gene Therapy
VP Protein Standards
We introduce PROGEN's recombinant VP standards used with Simple Western automated capillary electrophoresis in fit-for-purpose solutions for viral titer and VP capsid protein ratio measurements.
Advanced AAV Analytics
Cell and Gene Therapy Catapult and Simple Western team up for advanced AAV vector characterization, addressing challenges in assay precision, standardization, and throughput, enabling in-line product characterization.
Other Cell & Gene Therapy Resources from Bio-Techne
Customer Success Stories
Early Development Scientist, Oxford Biomedica
Senior Postdoctoral Researcher, University of Oxford
Centre for Tissue Engineering & Regenerative Medicine, UKM