Our Partnership with Spear Bio
At Bio-Techne, we believe collaboration drives scientific innovation, equipping researchers with the tools needed to advance disease understanding and therapeutic development. With that vision in mind, we have partnered with Spear Bio to distribute next-generation ultra-sensitive immunoassays, enabling the detection of low-abundance biomarkers in neurology and other disease areas.
This partnership brings together Bio-Techne’s global reach and immunoassay expertise with Spear Bio’s breakthrough platform, offering unmatched sensitivity for detecting key disease biomarkers. Together, we are advancing the future of biomarker detection, enabling discoveries that drive scientific and medical progress.
Meet SPEAR UltraDetect
Successive Proximity Extension Amplification Reaction (SPEAR) technology overcomes the limitations of conventional proximity extension assays and dramatically reduces transient noise to achieve ultrasensitive and biomarker detection. By integrating nucleic acid amplification into a unique two-factor authentication mechanism for protein detection, SPEAR enables highly specific and robust protein detection in a fully homogeneous, wash-free format using 1 µL of diluted sample.
SPEAR UltraDetect™ Immunoassays employ a simple and scalable workflow, easily adopted by any lab utilizing standard qPCR.
SPEAR UltraDetect Solution
The SPEAR UltraDetect Platform provides a comprehensive solution for biomarker measurement across diverse research and drug development applications.
Assay & Reaction Kits
Assay & Reaction Kits
Explore our growing menu of well-characterized single plex assays in the neurology space for the optimized detection of key blood-based biomarkers.
Benefits of the SPEAR UltraDetect Solution
1. Unparalleled Sensitivity
- Attomolar biomarker measurement with 1 µL of diluted sample.
2. Unparalleled Specificity
- Two-factor authentication ensures target-specific signal.
- No false positives from non-specific binding to a solid surface.
3. Unparalleled Precision
- Easy-to-automate workflow with <5% mean CV from 1 µL of diluted sample.
4. Unparalleled Robustness
- Highly consistent measurements across different qPCR platforms and formats.
A Homogeneous Assay Without Wash Steps
Step 1: Target Binding
Step 1: Target Binding
Incubate 1 μL of diluted sample with proprietary SPEAR probes, allowing sufficient time for antibodies to bind to their target.
Step 2: Two Factor Authentication Reaction
Step 2: Two Factor Authentication Reaction
Add reaction mix to the well without any washing. Two-factor authentication occurs, the key to achieving unparalleled sensitivity and specificity.
Step 3: Signal Amplification
Step 3: Signal Amplification
After the reaction is complete, qPCR mix is added to the well without any washing. After amplification, target-specific signal is quantified from the qPCR results.
Proven Sensitivity Advantage
SPEAR offers superior sensitivity compared to the most sensitive heterogeneous platforms.
What’s more, SPEAR Technology has less stringent requirements on antibody affinity and can consistently achieve high sensitivity across a wide range of biomarkers.
As seen in the figure on the left, SPEAR consistently detected analytes at sub-femtomolar concentrations, achieving LODs 2-6 orders of magnitude lower than the digital ELISA, even when low-affinity antibodies were employed.