DC-Cholesterol hydrochloride

Name: DC-Cholesterol hydrochloride
Brand: Tocris Bioscience
SKU: 7176

Tocris Bioscience, part of Bio-Techne | Catalog # 7176

COA / SDS

Cationic cholesterol derivative

Key Product Details

Description:	Cationic cholesterol derivative
Chemical Name:	(3β)-3-[N-[2-(Dimethylamino)ethyl]carbamate cholest-5-en-3-ol hydrochloride
Purity:	≥95% (HPLC)
Molecular Weight:	537.27

View Full Technical Details for DC-Cholesterol hydrochloride

Technical Data
Citations

Biological Activity for DC-Cholesterol hydrochloride

DC-Cholesterol hydrochloride is a cationic cholesterol derivative for use as a liposomal transfection reagent. Combines with the 'helper lipid' DOPE (Cat. No. 7175) after sonication or microfluidization to form unilamellar liposomes. Can be used in vitro and in vivo for transfer of nucleic acids, protein complexes, or other small molecule complexes into tissues, tumors and cells.

Technical Data for DC-Cholesterol hydrochloride

M. Wt	537.27
Formula	C₃₂H₅₆N₂O₂.HCl
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	166023-21-8
PubChem ID	16219102
InChi Key	ISXSJGHXHUZXNF-LXZPIJOJSA-N
Smiles	[H][C@]1([C@@H](CCCC(C)C)C)CC[C@]2([C@@]3(CC=C4C[C@@H](OC(NCCN(C)C)=O)CC[C@@]4([C@]3(CC[C@]12C)[H])C)[H])[H].Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DC-Cholesterol hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	2.69	5 with sonication
	ethanol	10.75	20

Preparing Stock Solutions for DC-Cholesterol hydrochloride

The following data is based on the product molecular weight 537.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	9.31 mL	46.53 mL	93.06 mL
1 mM	1.86 mL	9.31 mL	18.61 mL
2 mM	0.93 mL	4.65 mL	9.31 mL
10 mM	0.19 mL	0.93 mL	1.86 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for DC-Cholesterol hydrochloride

References are publications that support the biological activity of the product.