DC-Cholesterol hydrochloride
Tocris Bioscience, part of Bio-Techne | Catalog # 7176
Cationic cholesterol derivative
Key Product Details
Description
Cationic cholesterol derivative
Product Description
DC-Cholesterol hydrochloride is a cationic cholesterol derivative for use as a liposomal transfection reagent. Combines with the 'helper lipid' DOPE (Cat. No. 7175) after sonication or microfluidization to form unilamellar liposomes. Can be used in vitro and in vivo for transfer of nucleic acids, protein complexes, or other small molecule complexes into tissues, tumors and cells.Product Specifications
Molecular Weight
537.27
Formula
C32H56N2O2.HCl
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(3β)-3-[N-[2-(Dimethylamino)ethyl]carbamate cholest-5-en-3-ol hydrochloride
CAS Number
166023-21-8
PubChem ID
16219102
InChI Key
ISXSJGHXHUZXNF-LXZPIJOJSA-N
SMILES
[H][C@]1([C@@H](CCCC(C)C)C)CC[C@]2([C@@]3(CC=C4C[C@@H](OC(NCCN(C)C)=O)CC[C@@]4([C@]3(CC[C@]12C)[H])C)[H])[H].Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 2.69 | 5 with sonication | |
| Ethanol | 10.75 | 20 |
Preparing Stock Solutions
for DC-Cholesterol hydrochloride
The following data is based on the product molecular weight 537.27
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 9.31 mL | 46.53 mL | 93.06 mL |
| 1 mM | 1.86 mL | 9.31 mL | 18.61 mL |
| 2 mM | 0.93 mL | 4.65 mL | 9.31 mL |
| 10 mM | 0.19 mL | 0.93 mL | 1.86 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Li DC-Chol lipid system in gene transfer. J.Control.Release
Product Specific Notices for DC-Cholesterol hydrochloride
For research use only
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