1M7

Name: 1M7
Brand: Tocris Bioscience
SKU: 6602

Tocris Bioscience, part of Bio-Techne | Catalog # 6602

COA / SDS

Reagent for RNA SHAPE-MaP chemistry

Key Product Details

Description:	Reagent for RNA SHAPE-MaP chemistry
Alternative Names:	1-Methyl-7-nitroisatoic anhydride
Chemical Name:	1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione
Purity:	≥95% (HPLC)
Molecular Weight:	222.15

View Full Technical Details for 1M7

Technical Data
Citations

Biological Activity for 1M7

1M7 is a reagent for RNA SHAPE-MaP experiments. Enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.

Technical Data for 1M7

M. Wt	222.15
Formula	C₉H₆N₂O₅
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	73043-80-8
PubChem ID	12535373
InChi Key	MULNCJWAVSDEKJ-UHFFFAOYSA-N
Smiles	CN1C(OC(C2=CC=C([N+]([O-])=O)C=C12)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 1M7

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	22.21	100

Preparing Stock Solutions for 1M7

The following data is based on the product molecular weight 222.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.50 mL	22.51 mL	45.01 mL
5 mM	0.90 mL	4.50 mL	9.00 mL
10 mM	0.45 mL	2.25 mL	4.50 mL
50 mM	0.09 mL	0.45 mL	0.90 mL

Molarity Calculator

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Reconstitution Calculator

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References for 1M7

References are publications that support the biological activity of the product.

Smola Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 2015 PMID: 26544910
Turner Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 2013 PMID: 24141619
Siegfried RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 2014 PMID: 25028896
Mortimer A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 2007 PMID: 17367143

1M7