1M7
Tocris Bioscience, part of Bio-Techne | Catalog # 6602
Reagent for RNA SHAPE-MaP chemistry
Key Product Details
Description
Reagent for RNA SHAPE-MaP chemistry
Alternative Names
1-Methyl-7-nitroisatoic anhydride
Product Description
1M7 is a reagent for RNA SHAPE-MaP experiments. Enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.Product Specifications
Molecular Weight
222.15
Formula
C9H6N2O5
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione
CAS Number
73043-80-8
PubChem ID
12535373
InChI Key
MULNCJWAVSDEKJ-UHFFFAOYSA-N
SMILES
CN1C(OC(C2=CC=C([N+]([O-])=O)C=C12)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.21 | 100 |
Preparing Stock Solutions
for 1M7
The following data is based on the product molecular weight 222.15
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.50 mL | 22.51 mL | 45.01 mL |
| 5 mM | 0.90 mL | 4.50 mL | 9.00 mL |
| 10 mM | 0.45 mL | 2.25 mL | 4.50 mL |
| 50 mM | 0.09 mL | 0.45 mL | 0.90 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Smola Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 2015 PMID: 26544910
- Turner Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 2013 PMID: 24141619
- Siegfried RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 2014 PMID: 25028896
- Mortimer A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 2007 PMID: 17367143
Product Specific Notices for 1M7
For research use only
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