VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
Novus Biologicals, part of Bio-Techne | Catalog # H-1400-NB
Key Product Details
Applications
Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Microscopy
Product Summary for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.
VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.
Features:
Inhibits photobleaching of fluorescent dyes and fluorescent proteins Ideal refractive index (1.45) Ready-to-use No warming necessary Can be stored without sealing for long term analysis Hardening formulation No counterstain
VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.
Features:
Inhibits photobleaching of fluorescent dyes and fluorescent proteins Ideal refractive index (1.45) Ready-to-use No warming necessary Can be stored without sealing for long term analysis Hardening formulation No counterstain
Product Specifications
Application Notes
Refractive index is 1.46..
Scientific Data Images for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB] - VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - C6 rat glioma cells: Antibody to mitochondria localized with Alexa Fluor 555 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium. Image provided courtesy of Chandler Fulton and Jennifer Stella, Department of Biology, Brandeis University.Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB]
Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB] - VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - C6 rat glioma cells: Antibody to mitochondria localized with Alexa Fluor 555 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium. Photo taken after 15 minutes of irradiation under mercury vapor light source.Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark.
Background: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
Additional VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium Products
Product Documents for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
Product Specific Notices for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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