M.O.M.(R) (Mouse-On-Mouse) Staining Kit (Fluorometric)

Novus Biologicals, part of Bio-Techne | Catalog # FMK-2201-NB

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FMK-2201-NB
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Key Product Details

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Kit Type

Staining Kit (Fluorometric)

Product Summary for M.O.M.(R) (Mouse-On-Mouse) Staining Kit (Fluorometric)

Key advantages of the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Staining Kit (Fluorometric)
Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
Clear, crisp specific staining of antigens of interest
Based on VECTASTAIN(R) ABC reagent protocols
Procedures are simple and easy to follow
No tedious calculations or antibody prebinding steps required

Product Specifications

Application Notes

This Vector(R) M.O.M.(R) (Mouse-On-Mouse) kit contains sufficient stock reagents to provide approximately 25 ml of working solution which is usually enough to stain about 125-250 mouse tissue sections..

Kit Contents for M.O.M.(R) (Mouse-On-Mouse) Staining Kit (Fluorometric)

  • 0.1 ml M.O.M.(R) (Mouse-On-Mouse) Biotinylated Anti-Mouse IgG Reagent
  • 0.4 ml Fluorescein Avidin DCS
  • 1 ml M.O.M.(R) (Mouse-On-Mouse) Ig Blocking Reagent
  • 6 ml of M.O.M.(R) (Mouse-On-Mouse) Protein Concentrate

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: M.O.M.(R) (Mouse-On-Mouse)

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit is designed specifically to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. The background staining caused by the presence of endogenous mouse IgG can be essentially eliminated by using the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit. The M.O.M.(R) (Mouse-On-Mouse) Kit utilizes a novel blocking agent and a special detection methodology to significantly reduce this undesired background staining. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit can be used with normal and genetically engineered mouse models, including transgenic, xenograft, knock out and other mutant strains.

Additional M.O.M.(R) (Mouse-On-Mouse) Products

Product Documents for M.O.M.(R) (Mouse-On-Mouse) Staining Kit (Fluorometric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for M.O.M.(R) (Mouse-On-Mouse) Staining Kit (Fluorometric)

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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