SPEAR UltraDetect™ GFAP
Spear Bio | Catalog # SPR90010
SPEAR UltraDetect™ GFAP
Spear Assays
Successive Proximity Extension Amplification Reaction (SPEAR) technology overcomes the limitations of conventional proximity extension assays and dramatically reduces transient noise to achieve ultra-sensitive biomarker detection. This technology delivers rapid, precise, and reproducible results with minimal sample volume. SPEAR immunoassays employ a simple and scalable workflow, easily adopted by any lab utilizing standard qPCR.
Key Product Details
Sample Type & Volume Required Per Well
Serum (1 µL), EDTA Plasma (1 µL)
Sensitivity
0.163 pg/mL
Assay Range
0.625-20000 pg/mL (Serum, EDTA Plasma)
Product Summary for SPEAR UltraDetect™ GFAP
SPEAR UltraDetect Human GFAP (Glial Fibrillary Acidic Protein) was analytically verified for use with Human EDTA plasma and serum specimens.
Product Specifications
Reactivity
Human
Specificity
Natural and recombinant human GFAP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Apparently healthy volunteers were evaluated for the presence of human GFAP in this assay. No medical histories were available for the donors used in this study.
*Endogenous levels for heparin plasma and CSF were tested for reference only. Full verification of assay performance is suggested prior to conducting studies with these matrices.
| Sample Type (donors) | Mean Conc. (range) | Above LLoD % | Above LLoQ % |
| EDTA plasma (21 donors) | 37.5 pg/mL (8.0 - 77.2) | 100% | 100% |
| Serum (15 donors) | 33.6 pg/mL (8.9 - 57.5) | 100% | 100% |
| Heparin plasma* (10 donors) | 28.8 pg/mL (8.4 - 37.8) | 100% | 100% |
| CSF* (10 donors) | 3263 pg/mL (299 - 23535) | 100% | 100% |
Precision
Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | 1 | 1 | 2 | 2 | 3 | 3 | 1 | 1 | 2 | 2 | 3 | 3 |
| n | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| Mean (pg/mL) | 31.1 | 55.2 | 252 | 332 | 1535 | 3311 | 31.1 | 55.2 | 252 | 332 | 1535 | 3311 |
| Standard Deviation | ||||||||||||
| CV% | 2.8 | 1.1 | 1.2 | 1.0 | 2.2 | 1.8 | 6.4 | 4.9 | 3.9 | 4.7 | 5.7 | 5.5 |
Recovery for SPEAR UltraDetect™ GFAP
Recovery
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=3) | 103 | 98-108 |
| Serum (n=3) | 106 | 96-117 |
Linearity
To assess the accuracy of the assay, EDTA Plasma and Serum from apparently healthy donors were spiked with CSF and serially diluted 2x to 32x above assay MRD. Percent recovery was calculated as the sample concentration at higher dilution divded by the sample concentration at MRD.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: c-Abl
c-Abl (Abelson murine leukemia viral homolog 1) is a cytosolic member of the ABL subfamily, protein tyrosine kinase family of enzymes. It is ubiquitously expressed, and participates in multiple processes such as cell migration, actin reorganization, DNA damage response and apoptosis. Human c-Abl (I-B) is 1149 amino acids (aa) in length. It is myristoylated on Gly2 and contains one SH3 domain (aa 80-140), an SH2 domain (aa 146-236), a protein kinase region (aa 261-512), three NLS's (aa 620-634; 726-739; 778-791), one DNA-binding region (aa 888-988), an NES motif (aa 1109-1119) and an actin F-binding domain (aa 1120-1149). There is one alternate splice form (I-A) that contains a 26 aa substitution for the N-terminal 45 amino acids. In chronic myelogenous leukemia, c-Abl is fused to the Bcr gene product, resulting in the production of a constitutively active tyrosine kinase.
Long Name
Abelson Murine Leukemia Viral Oncogene Homolog 1
Alternate Names
ABL1, BCR-ABL1, bcr/abl, cAbl, JTK7
Gene Symbol
ABL1
Additional c-Abl Products
Product Documents for SPEAR UltraDetect™ GFAP
Product Specific Notices for SPEAR UltraDetect™ GFAP
For research use only
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