Topotecan hydrochloride
Tocris Bioscience, part of Bio-Techne | Catalog # 4562
DNA topoisomerase I inhibitor; Camptothecin (Cat. No. 1100) analog
Discontinued Product
4562 has been discontinued.
View all DNA Topoisomerase Inhibitors products.
Key Product Details
Description
DNA topoisomerase I inhibitor; Camptothecin (Cat. No. 1100) analog
Alternative Names
SK&F 104864-A
Product Description
Topotecan hydrochloride is a DNA topoisomerase I inhibitor; induces rapid apoptotic death in human B-lineage acute lymphoblastic leukemia (ALL) cells. Improves survival in three mouse models of poor prognosis ALL; downregulates B-Myb and MycN expression in neuroblastoma cells. Water-soluble analog of Camptothecin (Cat. No. 1100). Orally bioavailable. Topotecan disrupts the interaction between NHP2L1 and U4 RNA by binding to U4 and inhibits RNA splicing.Product Specifications
Molecular Weight
457.91
Formula
C23H23N3O5.HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
10-[(Dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1H-pyranoindolizino[1,2-b]quinoline-3,14(4H,12H)-dione hydrochloride
CAS Number
119413-54-6
PubChem ID
60699
InChI Key
DGHHQBMTXTWTJV-BQAIUKQQSA-N
SMILES
OC1=CC=C(N=C(C(N3C4)=CC([C@](CC)(O)C(OC5)=O)=C5C3=O)C4=C2)C2=C1CN(C)C.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 45.79 | 100 | |
| DMSO | 45.79 | 100 |
Preparing Stock Solutions
for Topotecan hydrochloride
The following data is based on the product molecular weight 457.91
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.18 mL | 10.92 mL | 21.84 mL |
| 5 mM | 0.44 mL | 2.18 mL | 4.37 mL |
| 10 mM | 0.22 mL | 1.09 mL | 2.18 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Sottile A chemical screen identifies the chemotherapeutic drug topo. as a specific inhibitor of the B-MYB/MYCN axis in neuroblastoma. Oncotarget. 2012 PMID: 22619121
- Schellens Bioavailability and pharmacokinetics of oral topotecan: a new topoisomerase I inhibitor. Br.J.Cancer 1996 PMID: 8630291
- Uckun In vitro and in vivo activity of topo. against human B-lineage acute lymphoblastic leukemia cells. Blood 1995 PMID: 7742543
Product Specific Notices for Topotecan hydrochloride
For research use only
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