TH 9619 disodium salt

Name: TH 9619 disodium salt
Brand: Tocris Bioscience
SKU: 7719

Tocris Bioscience, part of Bio-Techne | Catalog # 7719

COA / SDS

MTHFD2 inhibitor

Key Product Details

Description:	MTHFD2 inhibitor
Chemical Name:	Disodium N-[[5-[[2-(2,4-Diamino-1,6-dihydro-6-oxo-5-pyrimidinyl)acetyl]amino]-3-fluoro-2-pyridinyl]carbonyl]-L-glutamate
Purity:	≥98% (HPLC)
Molecular Weight:	495.33

View Full Technical Details for TH 9619 disodium salt

Technical Data
Citations

Biological Activity for TH 9619 disodium salt

TH 9619 disodium salt is a potent and selective methylenetetrahydrofolate dehydrogenase inhibitor (MTHFD2; IC₅₀ = 47 nM). It shows efficacity on HL-60 cell viability with EC₅₀ of 11nM. In vitro, It shows antiproliferative efficacy in acute myeloid leukemia (AML) cells and T-ALL Jurkat cells. In vivo, TH9619 disodium salt impairs cancer progression in a mouse disease model of AML.

Licensing Information

Sold under license from Thomas Helleday's Foundation for Medical Research and One-Carbon Therapeutics

Technical Data for TH 9619 disodium salt

M. Wt	495.33
Formula	C₁₇H₁₆FN₇Na₂O₇
Storage	Store at -20°C
Purity	≥98% (HPLC)
InChi Key	AKIUEXPYEGJOLL-WWPIYYJJSA-L
Smiles	NC1=NC(C(CC(NC2=CN=C(C(F)=C2)C(N[C@H](C(O[Na])=O)CCC(O[Na])=O)=O)=O)=C(N1)N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TH 9619 disodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	9.91	20 with gentle warming

Preparing Stock Solutions for TH 9619 disodium salt

The following data is based on the product molecular weight 495.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	10.09 mL	50.47 mL	100.94 mL
1 mM	2.02 mL	10.09 mL	20.19 mL
2 mM	1.01 mL	5.05 mL	10.09 mL
10 mM	0.20 mL	1.01 mL	2.02 mL

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References for TH 9619 disodium salt

References are publications that support the biological activity of the product.

Bonagas Pharmacological targeting of MTHFD2 suppresses acute myeloid leukemia by inducing thymidine depletion and replication stress. Nat.Cancer 2022 PMID: 35228749