Temozolomide is a DNA methylating, chemotherapeutic agent. Displays antitumor activity against a board spectrum of tumors, including leukemias, lymphomas and solid tumors (IC₅₀ = 5.0 μM for cytotoxicity against mouse TLX5 lymphoma cells). Induces autophagy in malignant glioma cells. Exhibits enhanced activity when used in combination with CHC (Cat No. 5029).

Product Specifications

Molecular Weight

194.15

Formula

C₆H₆N₆O₂

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3,4-Dihydro-3-methyl-4-oxoimidazo[5,1-d]-1,2,3,5-tetrazine-8-carboxamide

CAS Number

85622-93-1

PubChem ID

5394

InChI Key

BPEGJWRSRHCHSN-UHFFFAOYSA-N

SMILES

CN1N=NC2=C(N=CN2C1=O)C(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.71	50

Preparing Stock Solutions for Temozolomide

The following data is based on the product molecular weight 194.15

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	10.30 mL	51.51 mL	103.01 mL
2.5 mM	2.06 mL	10.30 mL	20.60 mL
5 mM	1.03 mL	5.15 mL	10.30 mL
25 mM	0.21 mL	1.03 mL	2.06 mL

Calculators

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

Background References

References are publications that support the biological activity of the product.

Kanzawa Role of autophagy in temozolomide-induced cytotoxicity for malignant glioma cells. Cell Death Differ. 2004 PMID: 14713959
Clark Antitumor imidazotetraazines. 32. Synthesis of novel imidazotetrazinones and related bicyclic heterocycles to probe the mode of action of the antitumor drug temozol. J.Med.Chem. 1995 PMID: 7739008
Bodell Formation of DNA adducts and induction of lacI mutations in big blue rat-2 cells treated with temozolomide: implications for the treatment of low-grade adult and pediatric tumors. Cancer Epidemiol.Biomarkers Prevent. 2003 PMID: 12815001
Plowman Preclinical antitumor activity of temozol. in mice: efficacy against human brain tumor xenografts and synergism with 1,3-bis(2-chloroethyl)-1-nitrosourea. Cancer Res. 1994 PMID: 8033099

Temozolomide

Tocris Bioscience, part of Bio-Techne | Catalog # 2706

Key Product Details

Description

Alternative Names

View all Apoptosis Inducer Compounds »

Product Description