S-Trityl-L-cysteine

Name: S -Trityl-L-cysteine
Brand: Tocris Bioscience
SKU: 2191
Rating: 4 (1 reviews)

Catalog # 2191 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (6)

COA / SDS

Potent, selective inhibitor of mitotic kinesin Eg5

Key Product Details

Description:	Potent, selective inhibitor of mitotic kinesin Eg5
Alternative Names:	NSC 83265
Chemical Name:	S-(Triphenylmethyl)-L-cysteine
Molecular Weight:	363.47

View Full Technical Details for S-Trityl-L-cysteine

Technical Data
Citations (6) / Reviews (1)

Biological Activity for S-Trityl-L-cysteine

S-Trityl-L-cysteine is a potent, cell-permeable, selective inhibitor of mitotic kinesin Eg5, a protein required for establishing and maintaining a bipolar spindle. Inhibits basal ATPase activity (IC₅₀ = 1 mM) and microtubule-activated ATPase activity of Eg5 (IC₅₀ = 140 nM). Induces mitotic arrest in HeLa cells with an IC₅₀ of 700 nM. Displays antitumor activity.

Technical Data for S-Trityl-L-cysteine

M. Wt	363.47
Formula	C₂₂H₂₁NO₂S
Storage	Store at +4°C
CAS Number	2799-07-7
PubChem ID	7271795
InChi Key	DLMYFMLKORXJPO-HXUWFJFHSA-N
Smiles	N[C@@H](C(O)=O)CSC(C2=CC=CC=C2)(C3=CC=CC=C3)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for S-Trityl-L-cysteine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	18.17	50

Preparing Stock Solutions for S-Trityl-L-cysteine

The following data is based on the product molecular weight 363.47.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.50 mL	27.51 mL	55.03 mL
2.5 mM	1.10 mL	5.50 mL	11.01 mL
5 mM	0.55 mL	2.75 mL	5.50 mL
25 mM	0.11 mL	0.55 mL	1.10 mL

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References for S-Trityl-L-cysteine

References are publications that support the biological activity of the product.

DeBonis In vitro screening for inhibitors of the human mitotic kinesin Eg5 with antimitotic and antitumour activities. Mol.Cancer Ther. 2004 PMID: 15367702
Brier Identification of the protein binding region of S-trityl-L-cysteine, a new potent inhibitor of mitotic kinesin Eg5. Biochemistry 2004 PMID: 15476401

Citations for S-Trityl-L-cysteine (6)

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Citations are publications that use Bio-Techne products. Selected citations for S-Trityl-L-cysteine include:

Cilibrasi et al. (2019) A Ploidy Increase Promotes Sensitivity of Glioma Stem Cells to Aurora Kinases Inhibition. J Oncol. 2019:9014045
PMID: 31531022
Ferreira et al. (2018) Dissecting the role of the tubulin code in mitosis. Methods Cell Biol. 144:33
PMID: 29804676
Hengeveld (2017) Inner centromere localization of the CPC maintains centromere cohesion and allows mitoticcheckpoint silencing. Nat Commun. 8:15542
PMID: 28561035
Hindriksen et al. (2017) Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells. PLoS One. 12:e0179514
PMID: 28640891
Müllers et al. (2014) Nuclear translocation of Cyclin B1 marks the restriction point for terminal cell cycle exit in G2 phase. PLoS Genet. 13:2733
PMID: 25486360
Hégarat et al. (2014) PP2A/B55 and Fcp1 regulate Greatwall and Ensa dephosphorylation during mitotic exit. J Pharmacol Exp Ther. 10:e1004004
PMID: 24391510

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Customer Reviews for S-Trityl-L-cysteine (1)

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STLC block cells at mitotic entry

Name: Valeria Marotta

Species: Human

Cell Line/Tissue: U2OS

I used the product to synchronise the cells at mitotic entry.

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