Rapamycin
Tocris Bioscience, part of Bio-Techne | Catalog # 1292
mTOR inhibitor; immunosuppressant
Product Description
Rapamycin is an antifungal and immunosuppressant. Rapamycin is a specific inhibitor of mTOR (mammalian target of rapamycin); complexes with FKBP-12 and binds mTOR inhibiting its activity. Rapamycin inhibits interleukin-2-induced phosphorylation and activation of p70 S6 kinase and induces autophagy in yeast and mammalian cell lines. Rapamycin drives hPSC differentiation to mesendoderm and blood progenitor cells. Also used as a chemical dimerizer; rapamycin and GA3-AM chemically inducible dimerization systems are orthogonal. Inhibits MERS-CoV infection of Huh7 cells in a plaque reduction assay. Enhances lentiviral transduction of hematopoietic stem cells.External Portal Information
Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of Rapamycin is reviewed on the chemical probes website.Product Specifications
Molecular Weight
914.18
Formula
C51H79NO13
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(3S,6R,7E,9R,10R,12R,14S,15E,17E,19E,21S,23S,26R,27R,34aS)-9,10,12,13,14,21,22,23,24,25,26,27,32,33,34,34a-Hexadecahydro-9,27-dihydroxy-3-[(1R)-2-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-methylethyl]-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-23,27-epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine-1,5,11,28,29(4H,6H,31H)-pentone
CAS Number
53123-88-9
PubChem ID
5284616
InChI Key
QFJCIRLUMZQUOT-HPLJOQBZSA-N
SMILES
O=C([C@H](C)\C=C([C@@H](O)[C@@H](OC)C([C@@H]4C)=O)/C)C[C@]([C@H](C)C[C@H]2C[C@@H](OC)[C@H](O)CC2)([H])OC([C@@]1([H])N(C(C([C@@]3(O)[C@H](C)CC[C@](C[C@@H](/C(C)=C/C=C/C=C/[C@H](C4)C)OC)([H])O3)=O)=O)CCCC1)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 18.28 | 20 |
Preparing Stock Solutions
for Rapamycin
The following data is based on the product molecular weight 914.18
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 5.47 mL | 27.35 mL | 54.69 mL |
| 1 mM | 1.09 mL | 5.47 mL | 10.94 mL |
| 2 mM | 0.55 mL | 2.73 mL | 5.47 mL |
| 10 mM | 0.11 mL | 0.55 mL | 1.09 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Wang Rapamycin relieves lentiviral vector transduction resistance in human and mouse hematopoietic stem cells Blood 2014 PMID: 24914132
- Galluzzi Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 2017 PMID: 28529316
- Kindrachuk Antiviral potential of ERK/MAPK and PI3K/AKT/mTOR signaling modulation for Middle East Respiratory Syndrome coronavirus infection as identified by temporal kinome analysis. Antimicrob.Agents Chemother. 2015 PMID: 25487801
- Miyamoto Rapid and orthogonal logic gating with a gibberellin-induced dimerization system Nat.Chem.Biol. 2012 PMID: 22446836
- Nazareth A multi-lineage screen reveals mTORC1 inhibition enhances human pluripotent stem cell mesendoderm and blood progenitor production. Stem Cell Reports 2016 PMID: 27132889
- Kuo Rapamycin selectively inhibits interleukin-2 activation of p70 S6 kinase. Nature 1992 PMID: 1614535
- Kobayashi Rapamycin, a specific inhibitor of the mammalian target of rapamycin, suppresses lymphangiogenesis and lymphatic metastasis. Cancer Sci. 2007 PMID: 17425689
- Huang Rapamycins: mechanism of action and cellular resistance. Cancer Biol.Ther. 2003 PMID: 12878853
Product Specific Notices for Rapamycin
For research use only
Loading...
Loading...
Loading...