Purvalanol B
Tocris Bioscience, part of Bio-Techne | Catalog # 1581
Selective cdk inhibitor; potently inhibits cdk1, cdk2 and cdk5
Key Product Details
Description
Selective cdk inhibitor; potently inhibits cdk1, cdk2 and cdk5
Alternative Names
NG 95
Product Description
Purvalanol B is a cyclin-dependent kinase (cdk) inhibitor (reported IC50 values are 6 nM for cdk1 and cdk5, and 6 - 9 nM for cdk2, depending on binding partner). Purvalanol B is selective over a range of other protein kinases (IC50 >10,000 nM). Purvalanol B shows antiproliferative properties, mediated by ERK1 and ERK2. Purvalanol B induces autophagy in cellular models and induces apoptosis in cancer cells, the apoptotic effects can be increased by combining with Rapamycin (Cat. No. 1292).Licensing Information
Sold under license from the Regents of the University of CaliforniaProduct Specifications
Molecular Weight
432.91
Formula
C20H25ClN6O3
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
(2R)-2-[[6-[(3-Chloro-4-carboxyphenyl)amino]-9-(1-methylethyl)-9H-purin-2-yl]amino]-3-methyl-1-butanol
CAS Number
212844-54-7
PubChem ID
448991
InChI Key
ZKDXRFMOHZVXSG-HNNXBMFYSA-N
SMILES
CC(C)[C@H](CO)NC1=NC(NC2=CC(Cl)=C(C=C2)C(O)=O)=C2N=CN(C(C)C)C2=N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 43.29 | 100 | |
| 1eq. NaOH | 43.29 | 100 with gentle warming |
Preparing Stock Solutions
for Purvalanol B
The following data is based on the product molecular weight 432.91
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.31 mL | 11.55 mL | 23.10 mL |
| 5 mM | 0.46 mL | 2.31 mL | 4.62 mL |
| 10 mM | 0.23 mL | 1.15 mL | 2.31 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.46 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Ozfiliz-Kilbas Cyclin-dependent kinase inhibitors, roscovitine and purvalanol, induce apoptosis and autophagy related to unfolded protein response in HeLa cervical cancer cells. Mol.Biol.Rep. 2018 PMID: 29978381
- Jorda How selective are pharmacological inhibitors of cell-cycle-regulating cyclin-dependent kinases? J.Med.Chem. 2018 PMID: 30234987
- Gray ATP-site directed inhibitors of cyclin-dependent kinases. Curr.Med.Chem. 1999 PMID: 10495356
- Knockaert p42/p44 MAPKs are intracellular targets of the CDK inhibitor purvalanol. Oncogene 2002 PMID: 12226745
- Gray Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors. Science 1998 PMID: 9677190
Product Specific Notices for Purvalanol B
For research use only
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