Poly(I:C)

Product Description

Poly(I:C) is a synthetic double-stranded RNA (dsRNA); endosomal Toll-like receptor 3 (TLR3) agonist. It also binds to the cytoplasmic receptors, retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA-5), elicits the secretion of type I interferon (IFN) and pro-inflammatory cytokines. Poly(I:C) is commonly used for immune system activation in vivo. Poly(I:C) also promotes maturation of hPSC-derived cardiomyocytes by modulating early Notch signaling and cardiomyogenic transcriptional programs. Poly(I:C) promotes generation of stable, mature dendritic cells in combination with TLR8 agonist CL 075 (Cat. No. 6142). Transfection into NIT-1 β cells induces β-cell apoptosis.

To prevent denaturation, this product should be reconstituted in solutions with physiological salt concentrations.

Solubility

Calculators

Background References

References are publications that support the biological activity of the product.

• Waele A systematic review on poly(I:C) and poly-ICLC in glioblastoma: adjuvants coordinating the unlocking of immunotherapy. J.Exp.Clin.Cancer Res. 2021 PMID: 34172082
• Biermann Epigenetic priming of human pluripotent stem cell-derived cardiac progenitor cells accelerates cardiomyocyte maturation. Stem Cells 2019 PMID: 31087611
• Spranger Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075. J.Immunol. 2010 PMID: 20511554
• West TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 2011 PMID: 21525932
• Rouas Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12. Int.Immunol. 2004 PMID: 15096480
• Robbins Nuclear factor-κB translocation mediates double-stranded ribonucleic acid-induced NIT-1 beta-cell apoptosis and up-regulates caspase-12 and tumor necrosis factor-associated ligand (TRAIL). Endocrinology 2003 PMID: 12960048