Phototrexate
Tocris Bioscience, part of Bio-Techne | Catalog # 7362
Photoswitchable inhibitor of human dihydrofolate reductase
Key Product Details
Description
Photoswitchable inhibitor of human dihydrofolate reductase
Product Description
Phototrexate is a photoswitchable potent inhibitor of human dihydrofolate reductase (DHFR). Phototrexate is a photochromic analog of Methotrexate (Cat. No. 1230). Antifolate activity is switched on (cis-Phototrexate) by UVA (375 nm) light; it can be switched off (trans-Phototrexate) by blue or white light and by thermal relaxation in the dark. cis-Phototrexate (IC50 = 6 nM) significantly reduces the viability of HeLa cells compared with trans-Phototrexate (IC50 = 34 μM).Scientific Data Images for Phototrexate
Photoswitching activity of Phototrexate
Antifolate activity is switched on (cis-Phototrexate) by UVA (375 nm) light; it can be switched off (trans-Phototrexate) by blue or white light and by thermal relaxation in the dark.Licensing Information
Sold with permission of IBEC.Product Specifications
Molecular Weight
437.42
Formula
C20H19N7O5
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N-[4-[(1E)-2-(2,4-Diamino-6-quinazolinyl)diazenyl]benzoyl]-L-glutamic acid
CAS Number
2268033-83-4
PubChem ID
142608675
InChI Key
IODLJULYGHWOLC-HIUCBWPRSA-N
SMILES
NC1=NC2=C(C(N)=N1)C=C(/N=N/C3=CC=C(C(N[C@H](C(O)=O)CCC(O)=O)=O)C=C3)C=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 1.75 | 4 |
Preparing Stock Solutions
for Phototrexate
The following data is based on the product molecular weight 437.42
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.04 mM | 57.15 mL | 285.77 mL | 571.53 mL |
| 0.2 mM | 11.43 mL | 57.15 mL | 114.31 mL |
| 0.4 mM | 5.72 mL | 28.58 mL | 57.15 mL |
| 2 mM | 1.14 mL | 5.72 mL | 11.43 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Mashita Light-wavelength-based quantitative control of dihydrofolate reductase activity by using a photochromic isostere of an inhibitor. Chembiochem. 2019 PMID: 30656808
- Matera Photoswitchable antimetabolite for targeted photoactivated chemotherapy. J.Am.Chem.Soc. 2018 PMID: 30346152
Product Specific Notices for Phototrexate
For research use only
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