PFI 3
Tocris Bioscience, part of Bio-Techne | Catalog # 5072
Potent and selective SMARCA2/4 and polybromo 1 inhibitor
Key Product Details
Description
Potent and selective SMARCA2/4 and polybromo 1 inhibitor
Product Description
PFI 3 is a potent and selective polybromo 1 (PBRM1) and SMARCA4 inhibitor (Kd values are 48 and 89 nM respectively) that also inhibits SMARCA2. This compoud displays 30-fold selectivity over other sub-family branches. PFI 3 accelerates FRAP recovery in cells at a concentration of 1 μM.Licensing Information
This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the PFI 3 probe summary on the SGC website.External Portal Information
Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of PFI 3 is reviewed on the chemical probes website.Product Specifications
Molecular Weight
321.37
Formula
C19H19N3O2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(2E)-1-(2-Hydroxyphenyl)-3-[(1R,4R)-5-(pyridin-2-yl)-2,5-diazabicyclo[2.2.1]heptan-2-yl]prop-2-en-1-one
CAS Number
1819363-80-8
PubChem ID
78243717
InChI Key
INAICWLVUAKEPB-QSTFCLMHSA-N
SMILES
O=C(C3=C(O)C=CC=C3)/C=C/N1[C@H](C4)CN(C2=NC=CC=C2)[C@H]4C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.14 | 100 | |
| Ethanol | 1.61 | 5 with gentle warming |
Preparing Stock Solutions
for PFI 3
The following data is based on the product molecular weight 321.37
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.11 mL | 15.56 mL | 31.12 mL |
| 5 mM | 0.62 mL | 3.11 mL | 6.22 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.11 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Gerstenberger Identification of a chemical probe for family VIII bromodomains through optimization of a fragment hit. J.Med.Chem. 2016 PMID: 27115555
- Vangamudi The SMARCA2/4 ATPase domain surpasses the bromodomain as a drug target in SWI/SNF mutant cancers: Insights from cDNA rescue and PFI-3 inhibitor studies Cancer Res. 2015 PMID: 26139243
Product Specific Notices for PFI 3
For research use only
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